I am installing Chameleon on my enclosed Prusa. I'm mounting filament spool holder to wall above and behind. My only question is whether I should feed the PTFE tubes in a bunch or spread them out. I've printed both options. The photo is just a draft of my bunch planned installation. I'm thinking spread out would be better.

Well, either will work fine... you just need to trim them to a shorter length. On the Prusa Mk3, I prefer to make two on one side and two on the other, that way the natural tendency for them is to hold up the Y adapter. I'd also shorten that distance between the Y and the extruder... no benefit to having it long. Look at my Mk3 video and you'll see how short I have it. It's a Mk2 on the Prusa, but the installation is identical: https://www.youtube.com/watch?v=LEJIslF33ew
Bill